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Cell analysis. optical imaging and spectroscopy. apoptosis. morphological phenotypes and patterns of gene expression during early cancer developmentOur research is focused on developing novel. minimally invasive optical technology to track signaling pathways and analyze the function of genes within living cells. Our work lies at the interface of optics and cell biology and combines instrumentation design. microscopy. tissue culture. genetic manipulation. and numerical simulation of optical phenomena. such as light scattering by cells. We are currently engaged in studying mitochondrial alterations in apoptosis. and defining optical parameters to characterize morphological alterations that result from abnormal expression of pro- or anti- apoptotic proteins. and abnormal mechanisms of cell death. One of our long-term goals is to understand the relationship between gene expression and subcellular structure and organization during early cancer development. Selected PublicationsPasternack RM, Qian Z, Zheng JY, Metaxas DN, Boustany NN. (2009) Highly sensitive size discrimination of sub-micron objects using optical Fourier processing based on two-dimensional Gabor filters. Opt Express. 17(14):12001-12. Pasternack RM, Qian Z, Zheng JY, Metaxas DN, White E, Boustany NN. (2008) Measurement of subcellular texture by optical Gabor-like filtering with a digital micromirror device. Opt Lett. 33(19):2209-11. Zheng JY, Tsai YC, Kadimcherla P, Zhang R, Shi J, Oyler GA, Boustany NN. (2008) The C-terminal transmembrane domain of Bcl-xL mediates changes in mitochondrial morphology. Boustany. N.N.. Tsai. Y.C.. Pfister. B.. Joiner. W.M.. Oyler. G.A.. Thakor. N.V. (2004). BCL-xL-dependent light scattering by apoptotic cells. Biophysical J. 87:(6) 4163-4171. Boustany. N.N.. rezek. R.. Thakor. N.V. (2002). Calcium-induced alterations in mitochondrial morphology quantified in situ with Optical Scatter Imaging. Biophysical J. 83:1691-1700. |
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