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Regulation of phospholipid metabolism. signaling in yeastDr. Carman’s research utilizes molecular genetic and biochemical approaches to study the regulation of phospholipid metabolism in the yeast Saccharomyces cerevisiae. Phospholipids are essential molecules that contribute to the structural definition of cell membranes, and participate in the regulation of cellular processes as signaling molecules and as reservoirs of lipid messengers. The Carman laboratory has made significant contributions to the understanding of phospholipid synthesis in yeast through the purification and characterization of several enzymes and the isolation and characterization of key genes. The laboratory has played a major role in the discovery that the expression of phospholipid biosynthetic enzymes is regulated by phospholipid precursors and the mineral zinc; and that key enzymes are regulated by membrane- and cytosolic-associated components and by covalent modification by protein kinases. These forms of enzyme regulation have profound effects on membrane phospholipid composition and have important medical implications for understanding the molecular basis for various diseases. Selected PublicationsMontero-Moran G, Caviglia JM, McMahon D, Rothenberg A, Subramanian V, Xu Z, Lara-Gonzalez S, Storch J, Carman GM, Brasaemle DL. (2009) CGI-58/ABHD5 is a coenzyme A-dependent lysophosphatidic acid acyltransferase. J Lipid Res. Oct 2. [Epub ahead of print] Elswaifi SF, St Michael F, Sreenivas A, Cox A, Carman GM, Inzana TJ. (2009) Molecular characterization of phosphorylcholine expression on the lipooligosaccharide of Histophilus somni.Microb Pathog. 47(4):223-30. Carman GM, Han GS. (2009) Regulation of phospholipid synthesis in yeast. J Lipid Res. 50 Suppl:S69-73. Carman GM, Han GS. (2009) Phosphatidic acid phosphatase, a key enzyme in the regulation of lipid synthesis. J Biol Chem. 284(5):2593-7. Grimsey, N., G. S. Han, L. O’ Hara, J. J. Rochford, G. M. Carman, and S. Siniossoglou. (2008). Temporal and spatial regulation of the phosphatidate phosphatases lipin 1 and 2. J. Biol. Chem. 283:29166-29174. Reprint Han, G.-S., L. O’ Hara, G.M. Carman, and S. Siniossoglou. (2008). An unconventional diacylglycerol kinase that regulates phospholipid synthesis and nuclear membrane growth. J. Biol. Chem. 283: 20433-20442. Reprint (JBC Paper of the Week) Han, G.-S., L. O’ Hara, S. Siniossoglou, and G.M. Carman. (2008). Characterization of the yeast DGK1-encoded CTP-dependent diacylglycerol kinase. J. Biol. Chem. 283: 20443-20453.Reprint (JBC Paper of the Week) Nadra, K., A.-S. de Preux Charles, J.-J. Médard, W.T. Hendriks, G.-S. Han, S. Grès, G.M. Carman, J.-S. Saulnier-Blache, M.H.G. Verheijen, and R. Chrast. (2008). Phosphatidic acid mediates demyelination in Lpin1 mutant mice. Genes Dev. 22:1647-1661. Reprint Soto, A., and Carman, G.M. (2008). Regulation of the Saccharomyces cerevisiae CKI1-encoded choline kinase by zinc depletion. J. Biol. Chem. 283: 10079-10088. Reprint Havriluk, T., Lozy, F., Siniossoglou, S. and Carman, G.M. (2008). Colorimetric determination of pure Mg2+-dependent phosphatidate phosphatase activity. Anal. Biochem. 373: 392-394. Reprint Han, G.-S., Siniossoglou, S. and Carman, G.M. (2007). The cellular functions of the yeast lipin homolog Pah1p are dependent on its phosphatidate phosphatase activity. J. Biol. Chem. 282: 37026-37035. Reprint Choi, H.-S., and Carman, G.M. ( 2007). Respiratory deficiency mediates the regulation of CHO1-encoded phosphatidylserine synthase by mRNA stability in Saccharomyces cerevisiae. J. Biol. Chem. 282: 31217-31227. Reprint Chang YF, Martin SS, Baldwin EP, Carman GM. (2007) Phosphorylation of human CTP synthetase 1 by protein kinase C: identification of Ser462 and Thr455 as major sites of phosphorylation. J. Biol. Chem. 282: 17613-17622. Reprint Choi MG, Carman GM. (2007) Phosphorylation of human CTP synthetase 1 by protein kinase A: identification of Thr455 as a major site of phosphorylation. J Biol Chem. 282(8):5367-77. Reprint ReviewsChang, Y.-F., and G.M. Carman. (2008). CTP synthetase and its role in phospholipid synthesis in the yeast Saccharomyces cerevisiae. Prog. Lipid Res. 47: 333-339. Reprint Carman, G.M., and S.A. Henry. (2007). Phosphatidic acid plays a central role in the transcriptional regulation of glycerophospholipid synthesis in Saccharomyces cerevisiae. J. Biol. Chem. 282: 37293-37297. Reprint Carman GM, Han GS. (2007) Regulation of phospholipid synthesis in Saccharomyces cerevisiae by zinc depletion. Biochim Biophys Acta. 1771(3):322-30. Reprint |
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