Isaac Kim
Assistant Professor

Roles of transforming growth factor- β and bone morphongenetic proteins in prostate cancer cells

The primary focus of our laboratory is the study of the tumor host interaction with the ultimate gial being the design of effective immunotherapy regimens for cancer. The interaction between the host immune system and tumor is a multifaceted one with the generation of productive immunity requiring the cooperation of immune cells from multiple lineages. Further complicating the system, tumor cells themselves can produce immune suppressive factors.

A focus of current studies in our laboratory lies in the development of genetic approaches towards immunotherapy. Recombinant vaccinia virus vectors are being explored as a means of transfecting tumor cells in-vivo with the ultimate goal of enhancing immunity. In this approach, viruses are engineered to contain the genes for immunologic helper factors or conversely neutralizing molecules such that when injected into tumors, they infect the tumor cells and cause the infected tumor to secrete the desired molecules.

Most recently, studies in out laboratory have included a new initiative towards the design of genetic vaccine strategies for breast cancer. Given an enhanced understanding of how immune responses are not only initiated but focused towards cell mediated or humoral (antibody producing) arms, we are in the process of "designing" genetic vaccines which will enhance the specific arms of an immune response. This latter focus will be greatly enhanced by the outstanding clinical and basic science resources focused on breast cancer at The Cancer Institute of New Jersey.

Selected Publications

Shen SS, Smith CL, Hsieh JT, Yu J, Kim IY, Jian W, Sonpavde G, Ayala GE, Younes M, Lerner SP. (2006) Expression of estrogen receptors-alpha and -beta in bladder cancer cell lines and human bladder tumor tissue. Cancer. 106(12):2610-6.

Abdelshehid, C, Ahlering, MT, Chou, D, Park, HK, Basillote, J, Lee, D, Kim, I, Eichel, L, Protsenko, D, Wong, B, McDougall, E, and Clayman, RV. (2005). Comparison of flexible ureteroscopes: deflection, irrigant flow and optical characteristics. Journal of Urology 173: 2017-2021.

Eichel, L, Kim, IY, Uribe, C, Khonsari, S, Basillote, J, Steward, E, Coad, J, Bischof, J, Rudie, E, Kluge, S, McDougall, EM and Clayman, RV. (2005) Comparison of radical nephrectomy, laparoscopic microwave thermotherapy, cryotherapy, and radiofrequency ablation for destruction of experimental VX-2 renal tumors in rabbits. Journal of Endourology 19: 1082-1087.

Kim, IY, Lee, DH, Lee, DK, Kim, WJ, Kim, MM, Morton, RA, Lerner, SP and Kim, SJ. (2004) Restoration of bone morphogenetic proteins receptor type II expression leads to a decreased rate of tumor growth in bladder transitional cell carcinoma cell line, TSU-Pr1. Cancer Research 64: 7355-7360.

Kim, IY, Lee, DH, Ahn, HJ, Kim, MM, Kim, SJ and Morton, RA. (2004) Loss of expression of bone morphogenetic protein receptor type II in human prostate cancer cells. Oncogene 23: 7651-7659.

Shariat, SF, Menesses-Diaz, A, Kim, IY, Muramoto, M, Wheeler, TM and Slawin, KM. (2004) Tissue expression of transforming growth factor-b1 and its receptors: correlation with pathologic features and biochemical progression in patients undergoing radical prostatectomy. Urology 63: 1191-1197.

Kim, IY, Lee, DH, Lee, DK, Kim, BC, Kim, HT, Leach, FS, Linehan, WM, Morton, RA and Kim, SJ. (2003) Decreased expression of bone morphogenetic protein receptor type II correlates with insensitivity to BMP-6 in human renal cell carcinoma cells. Clinical Cancer Research 9: 6046-6051.

Kim, IY, Kim, BC, Seong, DH, Lee, DK, Seo, JM, Kim, HT, Morton, RA and Kim, SK. (2002) Raloxifene, a mixed estrogen agonist/antagonist, induces apoptosis in androgen-independent human prostate cancer cell lines. Cancer Research 62: 5365-5369.

Kim, IY,Seong, DH, Kim, BC, Lee, DK, Remaley, AT, Leach, F, Morton, RA and Kim, SJ. (2002) Raloxifene, a selective estrogen receptor modulator, induces apoptosis in androgen-responsive human prostate cancer cell line, LNCaP, through an androgen-independent pathway. Cancer Research 62: 3649-3653.

Kim, HT,Kim, BC, Kim, IY, Mamura, M, Seong, DH, Jang, JJ and Kim, SJ. (2002) Raloxifene, a mixed estrogen agonist/antagonist, induces apoptosis through cleavage of BAD in human bladder cancer cells. Journal of Biological Chemistry 277: 32510-32515.

Lee, DK, Kim, BC, Kim, IY, Cho, EA, Satterwhite, DJ and Kim, SJ. (2002) The human papilloma virus E7 oncoprotein inhibits TGF-b signaling by blocking smad complex binding to its target sequence. Journal of Biological Chemistry 277: 38557-38564.

Shariat, SF, Kim, JH, Andrews, B, Kattan, MW, Wheeler, TM, Kim, IY, Lerner, SP and Slawin, KM. (2001) Preoperative plasma levels of transforming growth factor β1 strongly predict clinical outcome in patients with bladder carcinoma. Cancer 92: 2985-2992.

Kim, JH, Shariat, SF, Isaac Y. Kim, IY, Menesses-Diaz, A, Tokunaga, H, Wheeler, TMand Seth P. Lerner, SP. (2001) Predictive value of expression of transforming growth factor-beta(1) and its receptor in transitional cell carcinoma of the urinary bladder. Cancer 92: 1475-1483.

Young-Hyuck Im, Kim, HT, Kim, IY, Factor, VM, Hahm, KB, Anzano, M,Jang, JJ, Flanders, K, Haines, DC, Snorri S. Thorgeirsson, SS, Sizeland, A and Kim, SJ. (2001) Heterozygous mice for the transforming growth factor-b type II receptor gene have increased susceptibility to hepatocellular carcinogenesis. Cancer Research 61:6665-6668.

Shariat, SF, Shalev, M, Menesses-Diaz, A,Kim, IY, Kattan, MW, Wheeler, TM, and Slawin, KM. (2001) Preoperative plasma levels of transforming growth factor beta1 (TGF-b1) strongly predict progression in patients undergoing radial prostatectomy. Journal of Clinical Oncology 19: 2856-2864.

Kim, IY,Sadeghi, F, and Slawin, KM. (2001) Dyspareunia: an unusual presentation of leiomyoma of the bladder. Reviews in Urology 3: 152-154.

Kim, IY, Elliott, DS, Husmann, DA and Boone, TB. (2000) An unusual presenting symptom of sarcoidosis: neurogenic bladder dysfunction. Journal of Urology 165:903-904.

Kim, IY, Lee, DH, Ahn, HJ, Tokunaga, H, Song, W. Devereaux, LM, Jin, D, Sampath, TK and Morton, RA. (2000) Expression of bone morphogenetic protein receptors type-IA, -IB, and –II correlates with tumor grade in human prostate cancer tissues. Cancer Research 60: 2840-2844.

Kundu, S, Kim, IY, Yang, T, Doglio, L, Lang, S, Zhang, X, Buttyan, R, Kim, SJ, Chang, J, Cai, X, Wang, Z and Lee, C. (2000) Absence of proximal duct apoptosis in the ventral prostate of transgenic mice carrying the C3(1)-TGF-beta type II dominant negative receptor. Prostate 43: 118-124.

Kim, IY, Smith, C, Olivero, J and Lapin, SL. (2000) BCG peritonitis in a dialysis patient. Journal of Urology 163:237.