Ki-Bum Lee Assistant Professor Rutgers University Inst. for ADv Materials, Devices & Nanotech. Department of Chemistry & Chemical Biology Wright-Rieman Labs, 610 Taylor Rd. Piscataway. NJ 08854 (732) 445-2081 FAX - 5312 kblee@rutgers.edu Visit the Lee Lab

Chemical biology, Nanotechnology, Drug/Gene Delivery, Biomaterials/Tissue Engineering, Stem Cell Biology, Molecular Imaging, Synthetic Biology, and Functional Genomics

The primary research interest of my group at Rutgers is to develop and integrate nanotechnologies and chemical functional genomics to modulate signaling pathways in cells (e.g. stem cells and cancer cells) towards specific cell lineages or behaviors. In particular, my group is interested in studying how microenvironmental cues (e.g. soluble signals, cell-cell interactions, and insoluble/physical signals) functionally affect stem cell fate. To fully investigate the complex cell behaviors, we are developing both approaches from nanotechnology-the “top-down” pattering of extracellular matrix (ECM) and signal molecules in combinatorial ways (e.g. ECM compositions, pattern geometry, pattern density, and gradient patterns), and the “bottom-up” synthesis of multifunctional nanoparticles and their modification with specific signal molecules (e.g. siRNA and small organic molecules). Collectively, our research program is directly relevant to matters concerning biomaterials, nanomedicine, chemical biology and stem/cancer cell biology.

Selected Publications

Baik KY, Park SY, Heo K, Lee KB, Hong S. (2011) Carbon nanotube monolayer cues for osteogenesis of mesenchymal stem cells. Small. Mar 21;7(6):741-5.

Myung, S., Solanki, A., Kim, C., Park, J., Kim, K. S., Lee, K.-B.†, Graphene-encapsulated Nanoparticle-based Biosensor for the Selective Detection of Cancer Biomarkers", Advanced Materials, 2011 In Press. (†corresponding authors)

Kim, C., Shah, B. P., Subramaniam, P., Lee, K.-B.†, Cooperative induction of brain tumor cell apoptosis by targeted co-delivery of siRNA and anticancer drugs, Molecular Pharmaceutics, 2011 In Press. (†corresponding authors)

Park, J. K., Jung, J., Subramaniam, P., Shah, B., Kim, C., Lee, J. K., Cho, C., Lee, K.-B.†, Graphite-Coated Magnetic Nanoparticles as Multimodal Imaging Probes and Cooperative Therapeutic Agents for Tumor Cells, Small, 2011 In Press. (†corresponding authors)

Reyes, P. I., Ku, C.-J., Duan, Z., Lu, Y.†, Solanki A., Lee, K.-B.†, ZnO Thin Film Transistor Immunosensor with High Sensitivity and Selectivity, Applied Physics Letters, 2011 In Press. (†corresponding authors)

Baik, K. Y., Park, S. Y., Heo, K., Lee, K.-B., Hong, S, Carbon Nanotube Monolayer Cues for Osteogenesis of Mesenchymal Stem Cells, Small, 2011 In Press.

Jung, J., Solanki, A., Memoli, K. A., Kamei, K.-I., Kim, H. , Drahl, M. A., Williams, L. J., Tseng, H.-R., Lee, K.-B. (2010)†, Selective inhibition of human brain tumor cell proliferation via multifunctional quantum dot-based siRNA delivery, Angew. Chem. Int. Ed., 49, 103–107. (†:corresponding author)See also  Quantum dot based siRNA approach selectively inhibits brain cancer cells",http://www.nanowerk.com/spotlight/spotid=14396.php

Solanki A., Shah,S., Park, S. Y., Hong, S, Lee, K.-B.†, (2010) Controlling differentiation of neural stem cells using extracellular matrix protein patterns", Small 6:2509-2513. (Frontispiece in Small 22/2010)

Solanki, A., Lee, K.-B.†, (2010) A step closer to complete chemical reprogramming for generating iPS cells, ChemBioChem 11:755-757.

Solanki A, Lee KB. (2010) A step closer to complete chemical reprogramming for generating iPS cells. Chembiochem. Apr 12;11(6):755-7.

Jung, J., Solanki, A., Memoli, K. A., Kamei, K.-I., Kim, H. Drahl, M. A., Williams, L. J., Tseng, H.-R., and Lee, K-B† (2009) Selective inhibition of human brain tumor cell proliferation via multifunctional quantum dot-based siRNA delivery, accepted to Angew. Chem. Int. Ed. (†corresponding authors)

Brill, L. M.*, Xing W.*, Lee, K.-B.*, Ficarro, S.B., Xu, Y., Terskikh, A., Ding, S., (2009) Phosphoproteomic analysis of human embryonic stem cells. Cell Stem Cell 5:204-213. (* Equal First Authors.) See also Unraveling the human embryonic stem cell phosphoproteome. Cell Stem Cell 5:126-127 (Aug. 7, 2009).

Kamei, K.-I., Yu, Z. T. F., Guo, S., Takahashi, H., Gschweng, E., Wang, X., Suh, C., Tang, J., Witte, O. W.†, Lee, K.-B.† , Tseng, H.-R.† (2009) An integrated microfluidic device for reproducible and quantitative culture/assay of human embryonic stem cells. Lab Chip 9: 555-563. (†: corresponding authors)

Yu, Z. T. F., Kamei, K.-I., Shu, C. J., He, G. W., Silverman, R., Radu, C. G., Witte, O. W.†, Lee, K.-B.†, Tseng, H.-R.† (2009) An integrated microfluidic chip for parallel culture and multiparametric analysis of murine and human cell lines. Biomedical Microdevice 11: 547- 555. (†: corresponding authors)

Solanki, A., Kim, J. D., Lee, K.-B.† (2008) Nanotechnology for regenerative medicine: nanomaterials for stem cell imaging. Nanomedicine 3:567-578. (†: corresponding authors)

Lee, K.-B.† , Solanki, A., Kim, J. D., Jung J. (2008) Nanomedicine: dynamic integration of nanotechnology with biomedical science. Zhang, M., Editors, World Scientific. (†: corresponding authors)

Oh, B.-K, Park, S., Millstone, J. E., Lee, S. W., Lee, K.-B., Mirkin, C. A. (2006) Separation of tricomponent protein mixtures with Triblock Nanorods. J. Am. Chem. Soc. 128: 11825-11829.

Lee, K. -B., Kim, E. -Y., Wolinsky, S. M., Mirkin, C. A. (2004) The use of nanoarrays for highly sensitive and selective detection of human immunodeficiency virus in plasma. Nano Letters 4:1869-1872. See also "Nanoarrays for ultrasensitive biodetection", NanoToday", 9 (Dec. 2004).

Lee, K. -B., Park, S., Mirkin, C. A. (2004) Multicomponent magnetic nanorods for biomolecular separations. Angew. Chem. Int. Ed. 43:3048-3050.

Zhang, Y., Salaita, K., Lim, J -H., Lee, K. -B., Mirkin, C. A. (2004) A massively parallel electrochemical approach to the miniaturization of organic micro- and nanostructures on Surfaces. Langmuir 20:962-968.

Nam, J. -M., Han, S. W., Lee, K. -B., Liu, X., Mirkin, C. A. (2004) Bioactive protein nanoarrays on nickel oxide surfaces formed by Dip-Pen Nanolithography. Angew. Chem. Int. Ed. 43:2146-1249.

Zhang, H., Lee, K. -B., Li, Z., Mirkin, C. A. (2003) Biofunctionalized nanoarrays of inorganic structures prepared by dippen nanolithography. Nanotechnology 14:1113–1117.

Smith, J. C. *, Lee, K. -B.*, Wang, Q. *, Finn, M. G., Johnson, J. E., Mrksich, M., Mirkin, C. A. (2003) Nanopatterning the chemospecific immobilization of Cowpea Mosaic Virus Capsid. Nano Letters 3:883-886. (* These authors contributed equally to this work.)

Lim, J. -H., Ginger, D., Lee, K. -B., Heo, J., Nam, J. -M., Mirkin, C. A. (2003) Direct-Write Dip-Pen Nanolithography of proteins on modified silicon oxide surfaces Angew. Chem. Int. Ed. 20:2411-2414.

Lee, K. -B., Lim, J. -H., Mirkin, C. A. (2003) Protein nanostructures formed via Direct-Write Dip-Pen Nanolithography. J. Am. Chem. Soc. 125:5588-5589.