Daniel H. Shain
Associate Professor

Annelid development and evolution

Current research areas:

1. Cloning and expression of genes differentially expressed during embryonic stem cell formation.
2. Genetic and ultrastructural analyses of annelid cocoons
3. Bioenergetics of ice worms and extremophilic microbiota.
4. Ice worm phylogeny and phylogeography.

Selected Publications

Coleman J, Marotta R, Shain DH. (2008) Surface topology and structural integrity of the Theromyzon tessulatum (Annelida: Hirudinea: Glossiphoniidae) cocoon. J Morphol. 69(7):812-9.

Morrison BA, Shain DH. (2008) An AMP nucleosidase gene knockout in Escherichia coli elevates intracellular ATP levels and increases cold tolerance. Biol Lett. 4(1):53-6.

Marotta R, Shain DH. (2006) Irregular helicoids in leech cocoon membranes. J Struct Biol. 158(3):336-43.

Mason TA, McIlroy PJ, Shain DH. (2006) Structural model of an antistasin/notch-like fusion protein from the cocoon wall of the aquatic leech, Theromyzon tessulatum. J Mol Model. 12(6):829-34.

Dimitriu C. Sayers CW. Coleman J. Shain DH. (2006) Two-dimensional ultrastructural elements lead to three-dimensional reconstruction of protuberances on the cocoon membrane of the leech. Theromyzon tessulatum. Tissue Cell. 38(1):35-41.

Napolitano MJ. Shain DH.(2005) Quantitating adenylate nucleotides in diverse organisms. J Biochem Biophys Methods. 63(1):69-77.

Farrell AH. Hohenstein KA. Shain DH. (2004) Molecular adaptation in the ice worm. Mesenchytraeus solifugus: divergence of energetic-associated genes. J Mol Evol. 59(5):666-73.

Napolitano MJ. Shain DH. (2004) Four kingdoms on glacier ice: convergent energetic processes boost energy levels as temperatures fall. Proc Biol Sci. 271 Suppl 5:S273-6.

Napolitano MJ. Shain DH. (2005) Distinctions in adenylate metabolism among organisms inhabiting temperature extremes. Extremophiles. 9(2):93-8.

Shain DH. Stuart DK. Huang FZ. Weisblat DA. (2005) Cell interactions that affect axonogenesis in the leech Theromyzon rude. Development. x Sep;131(17):4143-53.

Hohenstein KA. Shain DH. (2004) Changes in gene expression at the precursor --> stem cell transition in leech. Stem Cells. 22(4):514-21.

Mason TA. McIlroy PJ. Shain DH. (2004) A cysteine-rich protein in the Theromyzon (Annelida: Hirudinea) cocoon membrane. FEBS Lett. 561(1-3):167-72.

Dimitriu C. Shain DH. (2004) Ultrastructural properties of the Theromyzon (Annelida: Hirudinae) cocoon membrane. Micron. 35(4):281-5.

Napolitano MJ. Nagele RG. Shain DH. (2004) The ice worm. Mesenchytraeus solifugus. elevates adenylate levels at low physiological temperature. Comp Biochem Physiol A Mol Integr Physiol. 137(1):227-35.